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A muscle biopsy (~100 mg) was collected at rest from the vastus lateralis muscle under local anesthesia (1% Xylocaine) using a Bergstrom needle modified for manual suction. Biopsies were quickly frozen in liquid nitrogen and stored at ?80 C until further analyses.

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Ferlin proteins mediate membrane-fusion events in response to Ca2+. Myoferlin, a member of the ferlin family, is required for normal muscle development, during which it mediates myoblast fusion. We isolated both damaged and intact myofibers from a mouse model of muscular dystrophy using laser-capture microdissection and found that the levels of

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